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Search results: MAP2K6 MEK6 MKK6

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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
Human MAP2K6 / MEK6 / MKK6 Protein (Recombinant GST, N-terminus) (aa231-334)LS-G59757
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Product group Lysates / Extracts
LifeSpan BioSciences
MAP2K6 / MEK6 / MKK6 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length)LS-G99859
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Product group Molecular Biology
SignalChem
MEK6 (MAP2K6), UnactiveM07-14G
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Product group Molecular Biology
SignalChem
MEK6 (MAP2K6), UnactiveM07-14H
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Product group Lysates / Extracts
LifeSpan BioSciences
Human MAP2K6 / MEK6 / MKK6 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length)LS-G74732
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Product group Assays
LifeSpan BioSciences
Mouse/Human/Rat Phospho-MAP2K6 / MEK6 / MKK6 (Ser207) (Cell-Based Phosphorylation ELISA) ELISA KitLS-F1567
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Product group Assays
RayBiotech
Human MKK6/MEK6 ELISA KitELH-MKK6
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Product group Assays
RayBiotech
Human/Mouse Phospho-MKK6 (S207) Cell-Based ELISA KitCBEL-MKK6
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Phospho-MKK6 (S207) ELISA Kit
Product group Assays
RayBiotech
Phospho-MKK6 (S207) ELISA KitPEL-MKK6-S207
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Phospho-MKK6 (S207) ELISA and Total MKK6 ELISA Kit
Product group Assays
RayBiotech
Phospho-MKK6 (S207) ELISA and Total MKK6 ELISA KitPEL-MKK6-S207-T
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Figure 1. Western blot analysis of MKK6/MAP2K6 using anti-MKK6/MAP2K6 antibody (A02011). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKK6/MAP2K6 antigen affinity purified polyclonal antibody (Catalog # A02011) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKK6/MAP2K6 at approximately 38 kDa. The expected band size for MKK6/MAP2K6 is at 38 kDa.
Product group Antibodies
Boster Bio
Anti-MEK6/MAP2K6 Antibody Picoband(r)A02011-BIOTIN
ApplicationsWestern Blot
TargetMAP2K6
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Figure 1. Western blot analysis of MKK6/MAP2K6 using anti-MKK6/MAP2K6 antibody (A02011). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKK6/MAP2K6 antigen affinity purified polyclonal antibody (Catalog # A02011) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKK6/MAP2K6 at approximately 38 kDa. The expected band size for MKK6/MAP2K6 is at 38 kDa.
Product group Antibodies
Boster Bio
Anti-MEK6/MAP2K6 Antibody Picoband(r)A02011-CARRIER-FREE
ApplicationsWestern Blot
TargetMAP2K6
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