Bio-Connect

Search results: MAPK11 p38 beta

193 to 204 of 197.700 products
Sort by
Western blot analysis of AIMP2 using anti-AIMP2 antibody (PA1481). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: Jurkat cell lysates, Lane 6: CEM cell lysates, Lane 7: HUT cell lysates, Lane 8: U93T cell lysates, Lane 9: U93T cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2 antigen affinity purified polyclonal antibody (Catalog # PA1481) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAIMP2
Product group Antibodies
Sino Biological
  • SizePrice