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Figure 2. Sandwich ELISA - Recombinant mouse MBL2 protein standard curve. Use in combination with reagents from Mouse MBL2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0806).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetMbl2
  • SizePrice
Figure 2. Sandwich ELISA - Recombinant mouse MBL2 protein standard curve. Use in combination with reagents from Mouse MBL2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0806).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA
ReactivityMouse, Rat
TargetMbl2
  • SizePrice
Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-5) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetMBL2
  • SizePrice
Figure 1. Western blot analysis of MBL2 using anti-MBL2 antibody (A01000-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBL2 antigen affinity purified polyclonal antibody (Catalog # A01000-5) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MBL2 at approximately 26 kDa. The expected band size for MBL2 is at 26 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetMBL2
  • SizePrice
Product group Molecular Biology
Sino Biological
  • SizePrice
Product group Molecular Biology
Sino Biological
  • SizePrice
Product group Proteins / Signaling Molecules
Sino Biological
  • SizePrice
Product group Proteins / Signaling Molecules
Sino Biological
  • SizePrice
Product group Molecular Biology
Sino Biological
  • SizePrice
Product group Molecular Biology
Abbexa
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Product group Molecular Biology
Abbexa
  • SizePrice
Product group Molecular Biology
Abbexa
  • SizePrice

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