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Search results: MCU

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Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Lysates / Extracts
LifeSpan BioSciences
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Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA873671LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
MCUB AntibodyCSB-PA873671LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetMCUB
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Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityMouse
TargetAreg
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Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetAreg
  • SizePrice
Figure 1. Western blot analysis of Amphiregulin using anti-Amphiregulin antibody (A01787-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin antigen affinity purified polyclonal antibody (Catalog # A01787-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin at approximately 41KD. The expected band size for Amphiregulin is at 28KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetAreg
  • SizePrice