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Search results: MCU

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Product group Antibodies
Everest Biotech
Goat anti-Fumarase / FHEB07874
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetFH
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.
Product group Antibodies
Boster Bio
Anti-FH Mouse Monoclonal Antibody [Clone ID: OTI1F10]M02097-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityCanine, Human, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-BIOTIN
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-CY3
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-DYLIGHT488
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-DYLIGHT550
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-DYLIGHT594
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-FITC
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-HRP
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-IFLUOR647
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
Figure 1. Western blot analysis of FH using anti-FH antibody (M02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: K562 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: COS-7 whole cell lysates, Lane 4: HL-60 whole cell lysates, Lane 5: Caco-2 whole cell lysates, Lane 6: U20S whole cell lysates, Lane 7: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FH antigen affinity purified monoclonal antibody (Catalog # M02097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for FH at approximately 48KD. The expected band size for FH is at 48KD.
Product group Antibodies
Boster Bio
Anti-FH Antibody Picoband(r) (monoclonal, 9D8)M02097-PE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetFH
  • SizePrice
4546474849

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