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Search results: MEKK2

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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityBovine, Canine, Equine, Human, Monkey, Mouse, Primate, Rat, Xenopus
TargetMAP3K2
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetMAP3K2
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
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Product group Antibodies
Biorbyt
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP3K2
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetMAP3K2
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Product group Antibodies
MedChem Express
TargetMAP3K2
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Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human THP-1 whole cell lysates Lane 4: human Raji whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityCanine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetMAP3K2
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Product group Molecular Biology
SignalChem
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityBovine, Canine, Equine, Human, Monkey, Mouse, Rat, Xenopus
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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