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Search results: NPC1

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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
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Product group Assays
LifeSpan BioSciences
ReactivityHuman
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Western blot analysis of Niemann Pick C1 using anti-Niemann Pick C1 antibody (A00428-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Niemann Pick C1 antigen affinity purified polyclonal antibody (Catalog # A00428-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Niemann Pick C1 at approximately 170KD. The expected band size for Niemann Pick C1 is at 142KD.
Product group Antibodies
Boster Bio
Product group Antibodies
Biorbyt
NPC1 antibodyORB100780
ApplicationsELISA
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Product group Antibodies
Biorbyt
NPC1 antibodyORB524629
ApplicationsELISA
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Product group Antibodies
Biorbyt
NPC1 antibodyORB381899
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Product group Antibodies
Biorbyt
NPC1 antibodyORB415305
ApplicationsELISA
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Product group Antibodies
Biorbyt
NPC1 antibodyORB412958
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
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Product group Lysates / Extracts
Sino Biological
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Product group Antibodies
Sino Biological
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