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Search results: NucSpot® Nuclear Stains

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Product group Chemicals
MyBioSource
Stains-AllMBS5755562
Estimated Purity98.00%
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Product group Chemicals
MyBioSource
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Product group Chemicals
MyBioSource
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Product group Chemicals
MyBioSource
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Product group Chemicals
MedChem Express
Stains-AllHY-D0987
CAS Number7423-31-6
Estimated Purity99.91
Molecular Weight559.58
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Product group Chemicals
Santa Cruz Biotechnology
CAS Number7423-31-6
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Product group Chemicals
Santa Cruz Biotechnology
CAS Number7423-31-6
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Product group Assays
Cosmo Bio USA
Product group Chemicals
MyBioSource
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Product group Antibodies
References
Nordic-MUbio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rabbit, Rat
TargetHNRNPK
Western blot analysis of THOC1 expression in HepG2 cell lysate using anti-THOC1 antibody (M04626). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THOC1 antigen affinity purified polyclonal antibody (Catalog # M04626) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for THOC1.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTHOC1
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetNFIA
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