More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/

Search results: OPN

49 to 60 of 1.675 products
Sort by
Product group Assays
DLdevelop
Rat Osteopontin (OPN) ELISA KitDL-OPN-Ra
  • SizePrice
Product group Assays
DLdevelop
Rabbit Osteopontin (OPN) ELISA KitDL-OPN-Rb
  • SizePrice
Product group Assays
DLdevelop
Human Osteopontin (OPN) ELISA KitDLR-OPN-Hu
  • SizePrice
Product group Assays
DLdevelop
Mouse Osteopontin (OPN) ELISA KitDLR-OPN-Mu
  • SizePrice
Product group Assays
DLdevelop
Rat Osteopontin (OPN) ELISA KitDLR-OPN-Ra
  • SizePrice
Product group Assays
DLdevelop
Rabbit Osteopontin (OPN) ELISA KitDLR-OPN-Rb
  • SizePrice
Anti-Osteopontin antibody, PA1431, Western blotting Lane 1: MM231 Cell Lysate Lane 2: HELA Cell Lysate Lane 3: JURKAT Cell Lysate Lane 4: COLO320 Cell Lysate
Product group Antibodies
Boster Bio
Anti-OPN AntibodyPA1431
Western blot analysis of Osteopontin using anti-Osteopontin antibody (RP1080). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human SHG-44 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osteopontin antigen affinity purified polyclonal antibody (Catalog # RP1080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Osteopontin at approximately 66,25-55KD. The expected band size for Osteopontin is at 35KD.
Product group Antibodies
Boster Bio
Anti-OPN AntibodyRP1080
Product group Antibodies
MyBioSource
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin)MBS648553
  • SizePrice
Product group Antibodies
References
Nordic-MUbio
Chicken anti human osteopontin-c (the shortest splice variant of osteopontin)AhOPNc
ApplicationsWestern Blot, ELISA
ReactivityHuman
Product group Antibodies
MyBioSource
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (Biotin)MBS6327834
  • SizePrice
Product group Antibodies
MyBioSource
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (FITC)MBS6327835
  • SizePrice
34567

Didn't find what you were looking for?

Search through our product groups to find the right product

Back to overview
AntibodiesChemicalsProteins / Signaling MoleculesAssaysDNA / RNA / VectorsCell CultureGenome Editing and EngineeringMolecular BiologyLysates / ExtractsExpressionLabware / AccesoriesBody ProductsTechnique Specific ProductsArrays / Slides