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Search results: PECAM1

49 to 60 of 1.149 products
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Product group Antibodies
Cusabio
PECAM1 AntibodyCSB-PA777657
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
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Product group Antibodies
Cusabio
PECAM1 AntibodyCSB-PA929097
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Product group Antibodies
Cusabio
PECAM1 AntibodyCSB-PA982317
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
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Product group Proteins / Signaling Molecules
Antibodies.com
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Product group Antibodies
Biorbyt
ApplicationsELISA
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Western blot analysis of CD31 using anti-CD31 antibody (A01513-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse ovary tissue lysates, Lane 2: mouse stomach tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # A01513-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 130KD. The expected band size for CD31 is at 82KD.
Product group Antibodies
Boster Bio
Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
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Western blot analysis of extracts of various cell lines, using Anti-PECAM1 Antibody (A11525) at 1:1,000 dilution.
Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution.
Lysates / proteins: 25µg per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Product group Antibodies
Antibodies.com
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Western blot analysis of extracts of THP-1 cells, using Anti-PECAM1 Antibody (A2104) at 1:500 dilution.
Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution.
Lysates / proteins: 25µg per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Product group Antibodies
Antibodies.com
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Western blot analysis of extracts of mouse liver, using Anti-PECAM1 Antibody (A0378) at 1:3000 dilution.
Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution.
Lysates / proteins: 25µg per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Product group Antibodies
Antibodies.com
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Product group Antibodies
Elabscience
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