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Search results: PLK2

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Western blot analysis of PLK2 using anti-PLK2 antibody (A02881).
Product group Antibodies
Boster Bio
Western blot analysis of PLK2/Snk using anti-PLK2/Snk antibody (PA2131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A431 Cell Lysate Lane 2: 293T Cell Lysate Lane 3: COLO-320 Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLK2/Snk antigen affinity purified polyclonal antibody (Catalog # PA2131) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLK2/Snk at approximately 78KD. The expected band size for PLK2/Snk is at 77KD.
Product group Antibodies
Boster Bio
Product group Chemicals
SignalChem
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Product group Chemicals
SignalChem
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Product group Proteins / Signaling Molecules
MyBioSource
PLK2, ActiveMBS517421
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus, Zebra Fish
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Molecular Biology
Sino Biological
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Product group Assays
LifeSpan BioSciences
ReactivityRat
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Product group Assays
LifeSpan BioSciences
ReactivityHuman
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