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Search results: RAF1

313 to 324 of 2.195 products
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Product group Antibodies
Biorbyt
ApplicationsELISA
ReactivityHuman
TargetRAF1
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Product group Antibodies
Biorbyt
ApplicationsELISA
ReactivityHuman
TargetRAF1
  • SizePrice
Product group Antibodies
Reddot Biotech
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetRAF1
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Product group Antibodies
Reddot Biotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAF1
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Western blot analysis of Raf1 expression in HeLa cell lysate (M00446). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody (Catalog # M00446) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAF1
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetRAF1
Western blot analysis of Raf1 expression in K562 cell lysate (M00446-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody (Catalog # M00446-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAF1
Product group Antibodies
References
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRAF1
WB analysis of HEK293 cells overexpressing c-Raf alone (Lane 1) or immunoprecipitates prepared from cells co-transfected with c-Raf, Ras (V12), and Rac1 (Lane 2-6) using GTX24768 Raf1 (phospho Ser338/Tyr340) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
Product group Antibodies
GeneTex
ApplicationsWestern Blot
ReactivityHuman
TargetRAF1
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Product group Antibodies
Sino Biological
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Product group Antibodies
Sino Biological
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice