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Search results: RAF1

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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group DNA / RNA / Vectors
OriGene
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Product group Molecular Biology
MedChem Express
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Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
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Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
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Western blot analysis of Phospho-Raf1 (S43) expression in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate (P00446-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAF1 monoclonal antibody (Catalog # P00446-3) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAF1
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetRAF1
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