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Figure 1. Western blot analysis of RALBP1 using anti-RALBP1 antibody (A01403-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALBP1 antigen affinity purified polyclonal antibody (Catalog # A01403-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALBP1 at approximately 95KD. The expected band size for RALBP1 is at 76KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetRALBP1
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
CategoryDNA / RNA / Vectors
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Product group Antibodies
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
TargetRALBP1
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Product group Antibodies
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetRALBP1
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