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Search results: RNF20

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Western blot analysis of RNF20 expression in (1) 293 cell lysate; (2) HeLa cell lysate (M03457-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF20 monoclonal antibody (Catalog # M03457-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF20
Product group Antibodies
Boster Bio
Western blot analysis of RNF20 in (1) MCF7 cell lysate; (2) HeLa cell lysate (M03457). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF20 monoclonal antibody (Catalog # M03457) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF20
Product group Antibodies
Boster Bio
Product group Molecular Biology
Sino Biological
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
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Product group Proteins / Signaling Molecules
Biorbyt
RNF20ORB599760
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Product group Proteins / Signaling Molecules
Cusabio
RNF20CSB-CL736198HU
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Product group DNA / RNA / Vectors
Sino Biological
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Product group Antibodies
Biorbyt
ApplicationsELISA
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Product group Antibodies
Biorbyt
ApplicationsELISA
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsELISA
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsELISA
  • SizePrice