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Search results: SAN-17001-EX/currency/EUR

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Product group (Cell) Culture
Cosmo Bio USA
FERTIUP((R)) Cryoprotectant: CPAKYD-001-05-EX-X5
Product group (Cell) Culture
Cosmo Bio USA
FERTIUP((R)) Preincubation Medium: PMKYD-002-05-EX-X5
Product group Labware / Accesories
Cosmo Bio USA
mHTFKYD-008-02-EX-X5
Product group Labware / Accesories
Cosmo Bio USA
mHTFKYD-008-05-EX-X3
Product group Labware / Accesories
Cosmo Bio USA
Crusher 10pcTKY-SK-100-D10-EX
Product group Labware / Accesories
Cosmo Bio USA
Crusher 100pcTKY-SK-100-D100-EX
Product group Labware / Accesories
Cosmo Bio USA
Automill 2mLX48 AdapterTKY-TK-AM7-48-EX
Product group Labware / Accesories
Cosmo Bio USA
PUREfrex 2.0PF201-0.25-EX
Product group Labware / Accesories
Cosmo Bio USA
PUREfrex((R))2.0GFK-PF201-0.25-EX
CgoaEWY7ND2EHlM6AAAAAJRWx6I999.png
Product group Chemicals
References
TargetMol Chemicals
San 201-456T34507
CAS Number79486-60-5
Molecular Weight931.14
Western blot analysis of UBE3A expression in K562 cell lysate using anti-UBE3A antibody (M00582). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE3A antigen affinity purified polyclonal antibody (Catalog # M00582) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for UBE3A.
Product group Antibodies
Boster Bio
Anti-E6AP Antibody (Monoclonal, EX-8)M00582
Product group Chemicals
Cell Guidance Systems
Wash Buffer (Concentrate 25x)Ex
  • SizePrice
2122232425

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