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Search results: SAV1

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Figure 1. Western blot analysis of SAV1 using anti-SAV1 antibody (A04183-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human DLD-1 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAV1 antigen affinity purified polyclonal antibody (Catalog # A04183-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAV1 at approximately 45 kDa. The expected band size for SAV1 is at 45 kDa.
Product group Antibodies
Boster Bio
Anti-SAV1 Antibody Picoband(r)A04183-2-PE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSAV1
  • SizePrice
Figure 1. Western blot analysis of SAV1 using anti-SAV1 antibody (A04183-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human DLD-1 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAV1 antigen affinity purified polyclonal antibody (Catalog # A04183-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAV1 at approximately 45 kDa. The expected band size for SAV1 is at 45 kDa.
Product group Antibodies
Boster Bio
Anti-SAV1 Antibody Picoband(r)A04183-2
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSAV1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SAV1 (N-term) Antibody102-22796
ApplicationsWestern Blot
ReactivityHuman
TargetSAV1
  • SizePrice
Product group Molecular Biology
Abbexa
Mouse SAV1 shRNA PlasmidABX975245
  • SizePrice
Product group Molecular Biology
Abbexa
Human SAV1 shRNA PlasmidABX961798
  • SizePrice
Product group Molecular Biology
Abbexa
Rat SAV1 shRNA PlasmidABX988975
  • SizePrice
Product group Antibodies
Abbexa
Protein Salvador Homolog 1 (SAV1) AntibodyABX029608
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
Abbexa
Protein Salvador Homolog 1 (SAV1) AntibodyABX135900
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
Abbexa
Protein Salvador Homolog 1 (SAV1) AntibodyABX321168
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Abbexa
Protein Salvador Homolog 1 (SAV1) AntibodyABX322004
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Assay Genie
[KO Validated] SAV1 AntibodyCAB18667
ReactivityHuman
TargetLY96
  • SizePrice
Product group Molecular Biology
Sino Biological
Human SAV1 qPCR Primer PairHP103384
  • SizePrice
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