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Search results: SIRT4

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Product group Molecular Biology
Sino Biological
Human, SIRT4 cDNA ORF Clone, C-Myc tagHG13076-CM
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Product group Molecular Biology
Sino Biological
Human, SIRT4 cDNA ORF Clone, C-HA tagHG13076-CY
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Product group Molecular Biology
Sino Biological
Human, SIRT4 cDNA ORF Clone in Cloning VectorHG13076-G
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Product group Molecular Biology
Sino Biological
Human, SIRT4 cDNA ORF Clone, N-His tagHG13076-NH
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Product group Molecular Biology
Sino Biological
Human, SIRT4 cDNA ORF Clone, N-Myc tagHG13076-NM
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Product group Molecular Biology
Sino Biological
Human, SIRT4 cDNA ORF Clone, N-HA tagHG13076-NY
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Figure 1. Western blot analysis of SIRT4 using anti-SIRT4 antibody (A03764-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT4 antigen affinity purified polyclonal antibody (Catalog # A03764-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT4 at approximately 38KD. The expected band size for SIRT4 is at 35KD.
Product group Antibodies
References
Boster Bio
Anti-SIRT4 Antibody Picoband(r)A03764-1
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetSIRT4
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Product group Molecular Biology
Abbexa
Mouse SIRT4 shRNA PlasmidABX978383
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Product group Molecular Biology
Abbexa
Human SIRT4 shRNA PlasmidABX958248
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Product group Antibodies
RayBiotech
Anti-SIRT4 (N-term) Antibody102-23091
ApplicationsWestern Blot
TargetSIRT4
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Product group Antibodies
Bioss
SIRT4 Polyclonal Antibody, APC ConjugatedBS-7537R-APC
ApplicationsWestern Blot
ReactivityBovine, Drosophila, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebra Fish
TargetSIRT4
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Product group Antibodies
Bioss
SIRT4 Polyclonal Antibody, Biotin ConjugatedBS-7537R-BIOTIN
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Drosophila, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Zebra Fish
TargetSIRT4
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