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Search results: SUPT5H

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Figure 1. Western blot analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPT5/SUPT5H antigen affinity purified polyclonal antibody (Catalog # A03426-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPT5/SUPT5H at approximately 150 kDa. The expected band size for SPT5/SUPT5H is at 121 kDa.
Product group Antibodies
Boster Bio
Anti-SPT5/SUPT5H Antibody Picoband(r)A03426-2-10UG
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSUPT5H
  • SizePrice
Figure 1. Western blot analysis of SPT5/SUPT5H using anti-SPT5/SUPT5H antibody (A03426-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPT5/SUPT5H antigen affinity purified polyclonal antibody (Catalog # A03426-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPT5/SUPT5H at approximately 150 kDa. The expected band size for SPT5/SUPT5H is at 121 kDa.
Product group Antibodies
Boster Bio
Anti-SPT5/SUPT5H Antibody Picoband(r)A03426-2-APC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetSUPT5H
  • SizePrice
Product group Molecular Biology
Sino Biological
Human, SUPT5H cDNA ORF Clone, C-GFPSpark(r) tagHG18941-ACG
  • SizePrice
Product group Molecular Biology
Sino Biological
Human, SUPT5H cDNA ORF Clone, C-OFPSpark(r) tagHG18941-ACR
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Product group Molecular Biology
Sino Biological
Human, SUPT5H cDNA ORF Clone, N-GFPSpark(r) tagHG18941-ANG
  • SizePrice
Product group Molecular Biology
Sino Biological
Human, SUPT5H cDNA ORF Clone, N-OFPSpark(r) tagHG18941-ANR
  • SizePrice
Product group Molecular Biology
Sino Biological
Human, SUPT5H cDNA ORF Clone, C-DYKDDDDK (Flag(r)) tagHG18941-CF
  • SizePrice
Product group Molecular Biology
Sino Biological
Human, SUPT5H cDNA ORF Clone, N-DYKDDDDK (Flag(r)) tagHG18941-NF
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SPT5 / SUPT5H AntibodyLS-C668098
ApplicationsWestern Blot
ReactivityHuman
TargetSUPT5H
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SPT5 / SUPT5H AntibodyLS-C410727
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetSUPT5H
  • SizePrice
Product group Antibodies
Abbexa
Transcription Elongation Factor SPT5 (SUPT5H) AntibodyABX124156
ApplicationsImmunoPrecipitation, Western Blot
  • SizePrice
Product group Antibodies
Abbexa
Transcription Elongation Factor SPT5 (SUPT5H) AntibodyABX145662
ApplicationsWestern Blot, ELISA
  • SizePrice
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