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Search results: Siglec-3

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Western blot analysis of CBX3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate using anti-CBX3 antibody (M01142-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX3 antigen affinity purified polyclonal antibody (Catalog # M01142-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CBX3.
Product group Antibodies
Boster Bio
Anti-HP1 gamma Rabbit Monoclonal AntibodyM01142-1
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCBX3
  • SizePrice
Western blot analysis of CD36 expression in 3T3 cell lysate.
Product group Antibodies
Boster Bio
Anti-CD36/Sr B3 Rabbit Monoclonal AntibodyM01189
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCD36
  • SizePrice
Western blot analysis of extracts from HeLa cells, using CDC7 antibody.
Product group Antibodies
Boster Bio
Anti-CDC7 Rabbit Monoclonal AntibodyM01190
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman, Mouse, Rat
TargetCDC7
  • SizePrice
Western blot analysis of CHAT expression in SH-SY5Y cell lysate.
Product group Antibodies
Boster Bio
Anti-CHAT/Choline Acetyltransferase Rabbit Monoclonal AntibodyM01192
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCHAT
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human tonsil, using CD9 Antibody.
Product group Antibodies
Boster Bio
Anti-CD9 Rabbit Monoclonal AntibodyM01202
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCD9
  • SizePrice
Western blot analysis of CYP1A2 expression in Caco2 cell lysate using anti-CYP1A2 antibody (M00598). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A2 antigen affinity purified polyclonal antibody (Catalog # M00598) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Product group Antibodies
Boster Bio
Anti-Cytochrome P450 1A2 CYP1A2 Monoclonal AntibodyM00598
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCYP1A2
  • SizePrice
Western blot analysis of CD68 expression in Jurkat cell lysate.
Product group Antibodies
Boster Bio
Anti-CD68/Sr D1 Rabbit Monoclonal AntibodyM00602-1
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCD68
  • SizePrice
Western blot analysis of RANTES expression in RANTES recombinant protein lysate.
Product group Antibodies
Boster Bio
Anti-RANTES CCL5 Monoclonal AntibodyM00617
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetCCL5
  • SizePrice
Western blot analysis of MCSF expression in MCSF recombinant protein (M00620). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSF1 monoclonal antibody (Catalog # M00620) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSF1
Product group Antibodies
Boster Bio
Anti-MCSF Rabbit Monoclonal AntibodyM00620
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCSF1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded human kidney, using Collagen I Antibody.
Product group Antibodies
References
Boster Bio
Anti-Collagen I COL1A1 Rabbit Monoclonal AntibodyM00624
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCOL1A1
  • SizePrice
Western blot analysis of CUL1 expression in MCF-7 cell lysate using anti-CUL1 antibody (M00658). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUL1 antigen affinity purified polyclonal antibody (Catalog # M00658) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CUL1.
Product group Antibodies
Boster Bio
Anti-Cullin 1 Monoclonal AntibodyM00658
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCUL1
  • SizePrice
Western blot analysis of CYP2E1 expression in HeLa cell lysate (M00672). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 monoclonal antibody (Catalog # M00672) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2E1
Product group Antibodies
References
Boster Bio
Anti-CYP2E1 Rabbit Monoclonal AntibodyM00672
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCYP2E1
  • SizePrice
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