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Search results: Siglec-3

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Western blot analysis of Raji cell lysate using CD22 antibody.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCD22
  • SizePrice
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCAV2
  • SizePrice
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCAV2
  • SizePrice
Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:100 dilution.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDH2
  • SizePrice
Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:100 dilution.
Product group Antibodies
References
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCDH2
  • SizePrice
Figure 1. Western blot analysis of Cullin4A using anti-Cullin4A antibody (M01579). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin4A antigen affinity purified monoclonal antibody (Catalog # M01579) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin4A at approximately 88 kDa. The expected band size for Cullin4A is at 88 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCUL4A
  • SizePrice
Figure 1. Western blot analysis of Cullin4A using anti-Cullin4A antibody (M01579). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin4A antigen affinity purified monoclonal antibody (Catalog # M01579) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin4A at approximately 88 kDa. The expected band size for Cullin4A is at 88 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCUL4A
  • SizePrice
Figure 1. Western blot analysis of Claudin 1 using anti-Claudin 1 antibody (M01585). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Claudin 1 antigen affinity purified monoclonal antibody (Catalog # M01585) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 1 at approximately 23 kDa. The expected band size for Claudin 1 is at 23 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCLDN1
  • SizePrice
Figure 1. Western blot analysis of Claudin 1 using anti-Claudin 1 antibody (M01585). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Claudin 1 antigen affinity purified monoclonal antibody (Catalog # M01585) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin 1 at approximately 23 kDa. The expected band size for Claudin 1 is at 23 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCLDN1
  • SizePrice
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCTSH
  • SizePrice
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCTSH
  • SizePrice
Western blot analysis of extracts from CCL19 recombinant protein, using CCL19 antibody.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman
TargetCCL19
  • SizePrice