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Search results: Siglec-3

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Western blot analysis of Casein Kinase 2 beta expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCSNK2B
  • SizePrice
Western blot analysis of Casein Kinase 2 beta expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCSNK2B
  • SizePrice
Western blot analysis of CD52 expression in Mouse spleen cell lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
ReactivityMouse
TargetCd52
  • SizePrice
Western blot analysis of CD52 expression in Mouse spleen cell lysate.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
ReactivityMouse
TargetCd52
  • SizePrice
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetCRYAB
  • SizePrice
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetCRYAB
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR8
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCCR8
  • SizePrice
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCYCS
  • SizePrice
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCYCS
  • SizePrice
Figure 1. Western blot analysis of CYCS using anti-CYCS antibody (M03529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HT-1080 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS antigen affinity purified monoclonal antibody (Catalog # M03529-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYCS at approximately 14 kDa. The expected band size for CYCS is at 12 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCYCS
  • SizePrice
Figure 1. Western blot analysis of CYCS using anti-CYCS antibody (M03529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HT-1080 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat skeletal muscle tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYCS antigen affinity purified monoclonal antibody (Catalog # M03529-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYCS at approximately 14 kDa. The expected band size for CYCS is at 12 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCYCS
  • SizePrice