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Search results: Siglec-3

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Figure 1. Western blot analysis of CD33 using anti-CD33 antibody (PB9932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SKOV3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD33 antigen affinity purified polyclonal antibody (Catalog # PB9932) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD33 at approximately 45 kDa. The expected band size for CD33 is at 40 kDa.
Product group Antibodies
Boster Bio
Anti-CD33 Antibody Picoband(r)PB9932-PE
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD33
  • SizePrice
Figure 1. Western blot analysis of CD33 using anti-CD33 antibody (PB9932). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: SKOV3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD33 antigen affinity purified polyclonal antibody (Catalog # PB9932) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD33 at approximately 45 kDa. The expected band size for CD33 is at 40 kDa.
Product group Antibodies
Boster Bio
Anti-CD33 Antibody Picoband(r)PB9932
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCD33
  • SizePrice
Figure 1. Western blotting validation for Anti-CD33 Antibody A01508-1 Western blot analysis of extracts of various cell lines
Product group Antibodies
Boster Bio
Anti-CD33 AntibodyA01508-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCD33
  • SizePrice
Western blot analysis of CD33 in 3T3 cell lysate with CD33 antibody at (A) 1 and (B) 2 microg/mL.
Product group Antibodies
Boster Bio
Anti-CD33 AntibodyA01508
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetCD33
  • SizePrice
Product group Antibodies
Boster Bio
Anti-GDF8 / Myostatin Reference Antibody (domagrozumab)M00926-5
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
ReactivityHuman, Monkey, Mouse
TargetCD33
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Siglec-3/CD33 Reference Antibody (Gemtuzumab)M01508-3
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
ReactivityHuman
TargetCD33
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Siglec-3 / CD33 Reference Antibody (vadastuximab talirine)M01508-4
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
ReactivityHuman, Monkey
TargetCD33
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Siglec-3 / CD33 Reference Antibody (Gemtuzumab ozogamicin)M01508-6
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
ReactivityHuman, Mouse, Rat
TargetCD33
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Siglec-3 / CD33 Reference Antibody (IMGN779)M01508-7
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
ReactivityHuman
TargetCD33
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Siglec-3 / CD33 Reference Antibody (vadastuximab)M01508-8
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
ReactivityHuman, Monkey
TargetCD33
  • SizePrice
Product group Antibodies
Boster Bio
Anti-Siglec-3 / CD33 Reference Antibody (gemtuzumab-CLM)M01508-9
ApplicationsFunctional Assay, Flow Cytometry, ELISA, Other Application
ReactivityHuman
TargetCD33
  • SizePrice
Product group Antibodies
Sino Biological
Recombinant Anti-Siglec-3/CD33 Antibody (APC), Rabbit Monoclonal12238-R001-A
ApplicationsFlow Cytometry
  • SizePrice
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