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Search results: Sin3a

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Western blot analysis of SIN3A expression in (1) K562 cell lysate; (2) RAW 264.7 cell lysate using anti-SIN3A antibody (M01203). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIN3A antigen affinity purified polyclonal antibody (Catalog # M01203) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for SIN3A.
Product group Antibodies
Boster Bio
Anti-mSin3A Monoclonal AntibodyM01203
Product group Antibodies
LifeSpan BioSciences
SIN3A Antibody (N-Terminus)LS-B3492
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rat
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
SIN3A Antibody Blocking PeptideLS-E6469
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SIN3A Antibody (Preservative Free)LS-C815772
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityMouse
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SIN3A Antibody (N-Terminus)LS-C815710
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
SIN3A Antibody Blocking PeptideLS-E11389
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Product group Antibodies
LifeSpan BioSciences
SIN3A Antibody (aa806-856)LS-C806194
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
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Product group Proteins / Signaling Molecules
Atlas Antibodies
PrEST Antigen SIN3AAPREST87485
Protein IDQ96ST3
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Product group Proteins / Signaling Molecules
Atlas Antibodies
PrEST Antigen SIN3AAPREST87952
Protein IDQ96ST3
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Product group Antibodies
MyBioSource
SIN3A (Paired Amphipathic Helix Protein Sin3a, Histone Deacetylase Complex Subunit Sin3a, Transcriptional Corepressor Sin3a) (HRP)MBS6154969
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Product group Antibodies
MyBioSource
SIN3A (Paired Amphipathic Helix Protein Sin3a, Histone Deacetylase Complex Subunit Sin3a, Transcriptional Corepressor Sin3a) (Biotin)MBS6144363
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Product group Antibodies
MyBioSource
SIN3A (Paired Amphipathic Helix Protein Sin3a, Histone Deacetylase Complex Subunit Sin3a, Transcriptional Corepressor Sin3a) (FITC)MBS6149666
  • SizePrice
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