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Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
Product group Antibodies
References
Boster Bio
Anti-Survivin/BIRC5 Antibody Picoband(r)A00379
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetBIRC5
  • SizePrice
Product group Antibodies
Biorbyt
Survivin Rabbit mAbORB1951682
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetBirc5
  • SizePrice
Product group Antibodies
Biorbyt
Survivin Rabbit mAbORB1923643
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetBirc5
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Survivin Antibody, Biotinylated130-10048B-50
ApplicationsELISA
TargetBIRC5
  • SizePrice
Product group Proteins / Signaling Molecules
Biomatik
Recombinant Human Survivin (Surv)RPU43934
ApplicationsWestern Blot, Other Application
Protein IDO15392
  • SizePrice
Product group Antibodies
Biomatik
Survivin (Surv) Polyclonal AntibodyCAU24790
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetBIRC5
  • SizePrice
Product group Antibodies
Biomatik
Survivin (Surv) Monoclonal AntibodyCAU29407
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetBIRC5
  • SizePrice
Product group Antibodies
Biomatik
Survivin (Surv) Monoclonal AntibodyCAU30764
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetBIRC5
  • SizePrice
Product group Antibodies
Biorbyt
Survivin Rabbit monoclonal antibodyORB2298260
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetBirc5
  • SizePrice
Product group Antibodies
Biorbyt
Anti-Survivin/BIRC5 AntibodyORB2622765
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetBirc5
  • SizePrice
Product group Antibodies
Biorbyt
Anti-Survivin/BIRC5 AntibodyORB2622766
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetBirc5
  • SizePrice
Product group Antibodies
Biorbyt
Anti-Survivin/BIRC5 AntibodyORB2622767
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetBirc5
  • SizePrice
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