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Search results: Survivin

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Product group Assays
Biomatik
Assay Sample Typeserum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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Product group Assays
Biomatik
Assay Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
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Product group Assays
References
Cloud-Clone Corp.
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Product group Assays
References
Cloud-Clone Corp.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Product group Antibodies
References
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetBIRC5
  • SizePrice
Western blot analysis of Survivin expression in Neuro-2a cell lysate (M00379-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Birc5 monoclonal antibody (Catalog # M00379-2) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Birc5
Product group Antibodies
References
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetBirc5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetBIRC5
  • SizePrice

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