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Search results: Survivin

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Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Survivin (Surv)RPC045HU01
ApplicationsWestern Blot, Other Application
Protein IDO15392
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Survivin (Surv)RPC045MU01
ApplicationsWestern Blot, Other Application
Protein IDO70201
  • SizePrice
Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (A00379). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # A00379) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
Product group Antibodies
References
Boster Bio
Anti-Survivin AntibodyA00379
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa.
Product group Antibodies
References
Boster Bio
Anti-survivin AntibodyPA1474
ApplicationsWestern Blot
ReactivityCanine, Human
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.
Product group Antibodies
References
Boster Bio
Anti-Survivin AntibodyRP1026
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetBIRC5
  • SizePrice
Figure 1. Western blot analysis of Survivin using anti-Survivin antibody (RP1052). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse SP2/0 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # RP1052) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.
Product group Antibodies
References
Boster Bio
Anti-Survivin AntibodyRP1052
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityMouse
TargetBirc5
  • SizePrice
Product group Molecular Biology
Sino Biological
Mouse, Survivin/BIRC5 cDNA ORF Clone, untaggedMG57331-UT
  • SizePrice
Product group Molecular Biology
Sino Biological
Human, Survivin/BIRC5 cDNA ORF Clone, untaggedHG10356-UT
  • SizePrice
Product group Antibodies
Biomatik
Survivin (Surv) Monoclonal AntibodyCAU30764
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityPorcine
TargetBIRC5
  • SizePrice
Product group Antibodies
RayBiotech
Anti-Survivin Antibody, Biotinylated130-10048B-50
ApplicationsELISA
ReactivityHuman
TargetBIRC5
  • SizePrice
Human Survivin ELISA Kit
Product group Assays
Antibodies.com
Human Survivin ELISA KitA705
Assay Sample TypeSerum, plasma, body fluids, tissue lysates, and cell culture supernatants.
ReactivityHuman
  • SizePrice
Mouse Survivin ELISA Kit
Product group Assays
Antibodies.com
Mouse Survivin ELISA KitA76202
Assay Sample TypePlasma, tissue homogenates and other biological fluids.
ReactivityMouse
  • SizePrice
56789

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