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Search results: TCP1

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Western blot analysis of CCT2 using anti-CCT2 antibody (PB9992). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT2 antigen affinity purified polyclonal antibody (Catalog # PB9992) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT2 at approximately 57KD. The expected band size for CCT2 is at 57KD.
Product group Antibodies
Boster Bio
Anti-TCP1 beta Picoband AntibodyPB9992
Western blot analysis of CCT8 using anti-CCT8 antibody (PB9993).
Product group Antibodies
Boster Bio
Anti-TCP1 theta Picoband AntibodyPB9993
IHC analysis of CCT4 using anti-CCT4 antibody (PB9927). CCT4 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT4 Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-TCP1 delta Picoband AntibodyPB9927
Western blot analysis of CCT5 using anti-CCT5 antibody (PB9928). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody (Catalog # PB9928) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT5 at approximately 60KD. The expected band size for CCT5 is at 60KD.
Product group Antibodies
Boster Bio
Anti-TCP1 epsilon Picoband AntibodyPB9928
IHC analysis of TCP1 using anti-TCP1 antibody (PB9826).
Product group Antibodies
Boster Bio
Anti-TCP1 Alpha Picoband AntibodyPB9826
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
Human TCP1 Protein (Recombinant His)LS-G991
  • SizePrice
GTX105725 IHC-P Image
Product group Antibodies
References
GeneTex
TCP1 theta antibody [N2C2], InternalGTX105725
  • SizePrice
Product group Antibodies
GeneTex
TCP1 alpha antibody, C-termGTX10180
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
  • SizePrice
Flow Cytometry analysis of HepG2 cells using anti-CCT7 antibody (A08169-2). Overlay histogram showing HepG2 cells stained with A08169-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT7 Antibody (A08169-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Product group Antibodies
Boster Bio
Anti-TCP1 eta/CCT7 AntibodyA08169-2
IHC analysis of TCP1 alpha using anti-TCP1 alpha antibody (M02389). TCP1 alpha was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-TCP1 alpha Antibody (M02389) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Anti-TCP1 alpha Antibody (monoclonal, 2E7)M02389
GTX75919 WB Image
Product group Antibodies
GeneTex
TCP1 eta antibody [PK/16/81a]GTX75919
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Rabbit
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CCT5 / TCP1 Epsilon Antibody (C-Terminus)LS-B11956
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Guinea Pig, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
  • SizePrice
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