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Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-CY3
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-DYLIGHT488
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-DYLIGHT550
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-DYLIGHT594
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-FITC
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-HRP
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-IFLUOR647
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462-PE
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Figure 1. Western blot analysis of BI-1/TMBIM6 using anti-BI-1/TMBIM6 antibody (A05462). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BI-1/TMBIM6 antigen affinity purified polyclonal antibody (Catalog # A05462) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BI-1/TMBIM6 at approximately 35 kDa. The expected band size for BI-1/TMBIM6 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-BI-1/TMBIM6 Antibody Picoband(r)A05462
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTMBIM6
  • SizePrice
Product group Molecular Biology
Abbexa
Human TMBIM1 shRNA PlasmidABX961928
  • SizePrice
Product group Molecular Biology
Abbexa
Mouse TMBIM1 shRNA PlasmidABX977007
  • SizePrice
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