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Figure 1. Western blot analysis of GHITM using anti-GHITM antibody (A11256-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHITM antigen affinity purified polyclonal antibody (Catalog # A11256-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHITM at approximately 37 kDa. The expected band size for GHITM is at 37 kDa.
Product group Antibodies
Boster Bio
Anti-GHITM Antibody Picoband(r)A11256-1-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGHITM
  • SizePrice
Figure 1. Western blot analysis of GHITM using anti-GHITM antibody (A11256-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHITM antigen affinity purified polyclonal antibody (Catalog # A11256-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GHITM at approximately 37 kDa. The expected band size for GHITM is at 37 kDa.
Product group Antibodies
Boster Bio
Anti-GHITM Antibody Picoband(r)A11256-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGHITM
  • SizePrice
Product group Antibodies
Boster Bio
Anti-GHITM AntibodyA11256
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetGHITM
  • SizePrice
Western blot analysis of extracts from LOVO cells, using GHITM antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
MICS1 (GHITM) Rabbit Polyclonal AntibodyTA315361
ApplicationsWestern Blot
ReactivityHuman
TargetGHITM
  • SizePrice
TA311419 (0.3ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product group Antibodies
OriGene
MICS1 (GHITM) Goat Polyclonal AntibodyTA311419
ApplicationsWestern Blot
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetGHITM
  • SizePrice
Immunohistochemistry of paraffin-embedded Human prostate cancer tissue using TA350175 (GHITM Antibody) at dilution 1/30 (Original magnification: x200)
Product group Antibodies
OriGene
MICS1 (GHITM) Rabbit Polyclonal AntibodyTA350175
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGHITM
  • SizePrice
Product group Antibodies
OriGene
MICS1 (GHITM) (117-127) Goat Polyclonal AntibodyAP23715PU-N
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Rat
TargetGHITM
  • SizePrice
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA364889 (GHITM Antibody) at dilution 1/30 (Original magnification: x200)
Product group Antibodies
OriGene
MICS1 (GHITM) Rabbit Polyclonal AntibodyTA364889
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGHITM
  • SizePrice
WB Suggested Anti-Ghitm Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Mouse Liver
Product group Antibodies
OriGene
MICS1 (GHITM) Rabbit Polyclonal AntibodyTA341936
ApplicationsWestern Blot
ReactivityMouse
TargetGHITM
  • SizePrice
Product group Antibodies
References
Everest Biotech
Goat anti-MICS1 / GHITMEB09815
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetGHITM
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-MICS1 / GHITM (aa117-127)EB09816
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Rat
TargetGHITM
  • SizePrice
Product group Antibodies
Bioss Antibodies
GRINA Polyclonal Antibody, Biotin ConjugatedBS-12098R-BIOTIN
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
TargetGRINA
  • SizePrice
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