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Figure 1. Western blot analysis of USP25 using anti-USP25 antibody (A06182-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey kidney tissue lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: human PC-12 whole cell lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse RAW264.7 whole cell lysates, Lane 14: mouse NIH-3T3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP25 antigen affinity purified polyclonal antibody (Catalog # A06182-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP25 at approximately 125KD. The expected band size for USP25 is at 125KD.
Product group Antibodies
Boster Bio
Anti-USP25 Antibody Picoband(r)A06182-1-PE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP25
  • SizePrice
Figure 1. Western blot analysis of USP25 using anti-USP25 antibody (A06182-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey kidney tissue lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: rat lung tissue lysates, Lane 11: human PC-12 whole cell lysates, Lane 12: mouse lung tissue lysates, Lane 13: mouse RAW264.7 whole cell lysates, Lane 14: mouse NIH-3T3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP25 antigen affinity purified polyclonal antibody (Catalog # A06182-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP25 at approximately 125KD. The expected band size for USP25 is at 125KD.
Product group Antibodies
Boster Bio
Anti-USP25 Antibody Picoband(r)A06182-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP25
  • SizePrice
Product group Antibodies
Enzo Life Sciences
USP21 (human) polyclonal antibodyBML-PW0585
  • SizePrice
Product group Antibodies
Enzo Life Sciences
USP21 (human) polyclonal antibodyBML-PW0585
  • SizePrice
Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
Product group Antibodies
Boster Bio
Anti-USP21 Antibody Picoband(r)A06639-1-BIOTIN
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP21
  • SizePrice
Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
Product group Antibodies
Boster Bio
Anti-USP21 Antibody Picoband(r)A06639-1-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP21
  • SizePrice
Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
Product group Antibodies
Boster Bio
Anti-USP21 Antibody Picoband(r)A06639-1-CY3
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP21
  • SizePrice
Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
Product group Antibodies
Boster Bio
Anti-USP21 Antibody Picoband(r)A06639-1-DYLIGHT488
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP21
  • SizePrice
Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
Product group Antibodies
Boster Bio
Anti-USP21 Antibody Picoband(r)A06639-1-DYLIGHT550
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP21
  • SizePrice
Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
Product group Antibodies
Boster Bio
Anti-USP21 Antibody Picoband(r)A06639-1-DYLIGHT594
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP21
  • SizePrice
Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
Product group Antibodies
Boster Bio
Anti-USP21 Antibody Picoband(r)A06639-1-FITC
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP21
  • SizePrice
Figure 1. Western blot analysis of USP21 using anti-USP21 antibody (A06639-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP21 antigen affinity purified polyclonal antibody (Catalog # A06639-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP21 at approximately 63KD. The expected band size for USP21 is at 63KD.
Product group Antibodies
Boster Bio
Anti-USP21 Antibody Picoband(r)A06639-1-HRP
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetUSP21
  • SizePrice
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