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Search results: Ubiquitin AMC

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Anti-Ubiquitin antibody, PA1420, Western blotting Lane 1: Rat Thymus Tissue Lysate Lane 2: Human MCF-7 Cell Lysate Lane 3: MM231 Cell Lysate Lane 4: HELA Cell Lysate Lane 5: SMMC Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetUBB
  • SizePrice
Anti-Ubiquitin antibody, PA1420, Western blotting Lane 1: Rat Thymus Tissue Lysate Lane 2: Human MCF-7 Cell Lysate Lane 3: MM231 Cell Lysate Lane 4: HELA Cell Lysate Lane 5: SMMC Cell Lysate
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetUBB
  • SizePrice
Figure 1. Western blot analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubiquitin antigen affinity purified polyclonal antibody (Catalog # PB9122) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubiquitin at approximately 9KD. The expected band size for Ubiquitin is at 9KD.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Canine, Chicken, Drosophila, Equine, Hamster, Human, Monkey, Mouse, Rabbit, Rat
TargetUBB
  • SizePrice
Figure 1. Western blot analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubiquitin antigen affinity purified polyclonal antibody (Catalog # PB9122) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubiquitin at approximately 9KD. The expected band size for Ubiquitin is at 9KD.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Canine, Chicken, Drosophila, Equine, Hamster, Human, Monkey, Mouse, Rabbit, Rat
TargetUBB
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
ApplicationsWestern Blot, Other Application
Protein IDQ93009
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Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
ApplicationsWestern Blot, Other Application
Protein IDQ569C3
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Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
ApplicationsWestern Blot, Other Application
Protein IDP40818
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Product group Antibodies
Cloud-Clone Corp.
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetUBD
  • SizePrice
Product group Antibodies
Cloud-Clone Corp.
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetUbd
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
ApplicationsWestern Blot, Other Application
Protein IDQ9NZ09
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Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
ApplicationsWestern Blot, Other Application
Protein IDO75604
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Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
ApplicationsWestern Blot, Other Application
Protein IDO88623
  • SizePrice

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