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Search results: Ubiquitin AMC

1741 to 1752 of 29.246 products
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Product group Chemicals
Biorbyt
Boc-LRR-AMC TFAORB2695159
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Product group Proteins / Signaling Molecules
Biorbyt
Boc-FAAGRK-AMC peptideORB216498
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Product group Proteins / Signaling Molecules
Biorbyt
Boc-GRR-AMC peptideORB216500
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Product group Proteins / Signaling Molecules
Biorbyt
Boc-PRR-AMC peptideORB216501
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Product group Proteins / Signaling Molecules
Biorbyt
Boc-RRR-AMC peptideORB216502
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Product group Proteins / Signaling Molecules
Biorbyt
Z-DEVD-AMC peptideORB216861
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Product group Chemicals
Biorbyt
Ac-Leu-Arg-AMCORB1679701
CAS Number929621-79-4
Estimated Purity98%
Molecular Weight486.56
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Product group Assays
MyBioSource
AMCMBS406371
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Figure 1. Western blot analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubiquitin antigen affinity purified polyclonal antibody (Catalog # PB9122) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubiquitin at approximately 9KD. The expected band size for Ubiquitin is at 9KD.
Product group Antibodies
Boster Bio
Anti-Ubiquitin/UBB Antibody Picoband(r)PB9122
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Canine, Chicken, Drosophila, Equine, Hamster, Human, Monkey, Mouse, Rabbit, Rat
TargetUBB
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Anti-Ubiquitin antibody, PA1420, Western blotting Lane 1: Rat Thymus Tissue Lysate Lane 2: Human MCF-7 Cell Lysate Lane 3: MM231 Cell Lysate Lane 4: HELA Cell Lysate Lane 5: SMMC Cell Lysate
Product group Antibodies
Boster Bio
Anti-Ubiquitin/UBB Antibody Picoband(r)PA1420
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetUBB
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Product group Chemicals
Biorbyt
Z-Val-Val-Arg-AMCORB2565008
CAS Number124485-41-2
Estimated Purity98.00%
Molecular Weight663.76
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Product group Chemicals
Biorbyt
Ala-Ala-Phe-AMC TFAORB2567055
Estimated Purity98.00%
Molecular Weight578.54
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