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Figure 1. Western blot analysis of ABCA4 using anti-ABCA4 antibody (PA2066). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # PA2066) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 280 kDa. The expected band size for ABCA4 is at 256 kDa.
Product group Antibodies
Boster Bio
Anti-ABCA4 Antibody Picoband(r)PA2066-IFLUOR647
ApplicationsWestern Blot
ReactivityMouse
TargetAbca4
  • SizePrice
Figure 1. Western blot analysis of ABCA4 using anti-ABCA4 antibody (PA2066). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # PA2066) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 280 kDa. The expected band size for ABCA4 is at 256 kDa.
Product group Antibodies
Boster Bio
Anti-ABCA4 Antibody Picoband(r)PA2066-PE
ApplicationsWestern Blot
ReactivityMouse
TargetAbca4
  • SizePrice
Figure 1. Western blot analysis of ABCA4 using anti-ABCA4 antibody (PA2066). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # PA2066) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 280 kDa. The expected band size for ABCA4 is at 256 kDa.
Product group Antibodies
Boster Bio
Anti-ABCA4 Antibody Picoband(r)PA2066
ApplicationsWestern Blot
ReactivityMouse
TargetAbca4
  • SizePrice
Product group Antibodies
Biorbyt
HLA Class 1 ABC/HLA ABC Rabbit pAb, PerCP-Cy5.5 conjugatedORB2444575
ApplicationsImmunoFluorescence
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • SizePrice
Product group Molecular Biology
LGC Biosearch Technologies
Stellaris(r) FISH Probes, Escherichia coli phosphate ABC transporter permease with Quasar(r) 570, 5 nmol TotalVSMF-9439-5
  • SizePrice
Product group Molecular Biology
LGC Biosearch Technologies
Stellaris(r) FISH Probes, Escherichia coli phosphate ABC transporter permease with Quasar(r) 670, 5 nmol TotalVSMF-9440-5
  • SizePrice
Product group Molecular Biology
LGC Biosearch Technologies
Stellaris(r) FISH Probes, Nematode ABC transporter, class F with CAL Fluor(r) Red 590, 5 nmol TotalVSMF-6209-5
  • SizePrice
Product group Molecular Biology
LGC Biosearch Technologies
Stellaris(r) FISH Probes, Nematode ABC transporter, class F with CAL Fluor(r) Red 610, 5 nmol TotalVSMF-6210-5
  • SizePrice
Product group Molecular Biology
LGC Biosearch Technologies
Stellaris(r) FISH Probes, Nematode ABC transporter, class F with CAL Fluor(r) Red 635, 5 nmol TotalVSMF-6213-5
  • SizePrice
Product group Molecular Biology
LGC Biosearch Technologies
Stellaris(r) FISH Probes, Nematode ABC transporter, class F with CAL Fluor(r) Orange 560, 5 nmol TotalVSMF-6214-5
  • SizePrice
Product group Assays
Biomatik
Human Abca7 (Atp-Binding Cassette Sub-Family A Member 7) ELISA KitEKF58899
Assay Sample TypeSerum, plasma, tissue homogenates and other biological fluids
ReactivityHuman
  • SizePrice
Product group Antibodies
Boster Bio
Anti-ABCAC ABCA12 AntibodyA03728
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetABCA12
  • SizePrice
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