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Figure 1. Western blot analysis of ACE2 using anti-ACE2 antibody (A00756-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE2 antigen affinity purified polyclonal antibody (Catalog # A00756-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE2 at approximately 100 kDa. The expected band size for ACE2 is at 92 kDa.
Product group Antibodies
Boster Bio
Anti-ACE2 Antibody Picoband(r)A00756-4
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetACE2
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Product group Molecular Biology
Sino Biological
Rhesus, ACE2 cDNA ORF Clone, C-His tagCG90211-CH
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Product group Molecular Biology
Sino Biological
Rhesus, ACE2 cDNA ORF Clone, C-Myc tagCG90211-CM
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Product group Molecular Biology
Sino Biological
Rhesus, ACE2 cDNA ORF Clone, C-HA tagCG90211-CY
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Product group Molecular Biology
Sino Biological
Rhesus, ACE2 cDNA ORF Clone in Cloning VectorCG90211-G
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Product group Molecular Biology
Sino Biological
Rhesus, ACE2 cDNA ORF Clone, N-His tagCG90211-NH
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Product group Molecular Biology
Sino Biological
Rhesus, ACE2 cDNA ORF Clone, N-Myc tagCG90211-NM
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Product group Molecular Biology
Sino Biological
Rhesus, ACE2 cDNA ORF Clone, N-HA tagCG90211-NY
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Product group Molecular Biology
Sino Biological
Human, ACE2 cDNA ORF Clone, C-His tagHG10108-CH
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Product group Molecular Biology
Sino Biological
Human, ACE2 cDNA ORF Clone, C-Myc tagHG10108-CM
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Product group Molecular Biology
Sino Biological
Human, ACE2 cDNA ORF Clone, C-HA tagHG10108-CY
  • SizePrice
Product group Molecular Biology
Sino Biological
Human, ACE2 cDNA ORF Clone in Cloning VectorHG10108-M
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