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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3.
Product group Antibodies
Boster Bio
Anti-AKT3 Mouse Monoclonal Antibody [Clone ID: OTI9B2]M00520-1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Figure 1. Western blotting validation for Anti-Akt3 Monoclonal Antibody M00520 Western Blot (WB) analysis using Akt3 Monoclonal Antibody against truncated Akt3 recombinant protein (1) and Human Ovary carcinoma tissue lysate (2). Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
Anti-Akt3 Monoclonal AntibodyM00520
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAKT3
  • SizePrice
Western blot analysis of AKT3 expression in GST Tagged recombinant AKT1 (lane 1), GST Tagged recombinant AKT2 (lane 2) and GST Tagged recombinant AKT3 (lane 3). AKT3 at 78KD was detected using mouse anti-AKT3 unconjugated monoclonal antibody at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
Product group Antibodies
Boster Bio
Anti-AKT3 FITC Monoclonal AntibodyF00520
ApplicationsDot Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using TA324153 (AKT3 Antibody) at dilution 1/30 (Original magnification: x200)
Product group Antibodies
OriGene
AKT3 Rabbit Polyclonal AntibodyTA324153
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Immunohistochemistry of paraffin-embedded Human brain tissue using TA324154 (AKT3 Antibody) at dilution 1/40 (Original magnification: x200)
Product group Antibodies
OriGene
AKT3 Rabbit Polyclonal AntibodyTA324154
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Western blot analysis of lysate from Jurkat cell line, using AKT3 Antibody (Q103) (Cat. #TA300042).TA300042 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Product group Antibodies
OriGene
AKT3 Rabbit Polyclonal AntibodyTA300042
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT3
  • SizePrice
Western blot analysis of AKT3 (arrow) using rabbit polyclonal AKT3 Antibody (S472) (Cat.#TA302230). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT3 gene (Lane 2) (Origene Technologies).
Product group Antibodies
OriGene
AKT3 Rabbit Polyclonal AntibodyTA302230
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetAKT3
  • SizePrice
TA302640 (1ug/ml) staining of HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Product group Antibodies
OriGene
AKT3 Goat Polyclonal AntibodyTA302640
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Rat
TargetAKT3
  • SizePrice
Western blot analysis of lysate from Jurkat cell line,using AKT3 Antibody (Q103).A00520-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Product group Antibodies
Boster Bio
Anti-AKT3 Antibody (Center)A00520-1
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT3
  • SizePrice
Western blot analysis of lysate from Jurkat cell line,using AKT3 Antibody (Q103).A00520-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Product group Antibodies
Boster Bio
Anti-AKT3 Antibody (Center)A00520-1
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Monkey, Mouse, Rat
TargetAKT3
  • SizePrice
Figure 1. Western blot analysis of AKT3 using anti-AKT3 antibody (A00520-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3 mouse brain tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (Catalog # A00520-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 60 kDa. The expected band size for AKT3 is at 56 kDa.
Product group Antibodies
Boster Bio
Anti-AKT3 Antibody Picoband(r)A00520-2-10UG
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
Figure 1. Western blot analysis of AKT3 using anti-AKT3 antibody (A00520-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3 mouse brain tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (Catalog # A00520-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 60 kDa. The expected band size for AKT3 is at 56 kDa.
Product group Antibodies
Boster Bio
Anti-AKT3 Antibody Picoband(r)A00520-2-APC
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetAKT3
  • SizePrice
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