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Search results: akt

2953 to 2964 of 11.329 products
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Product group Antibodies
OriGene
Phospho-AKT1 Rabbit Polyclonal AntibodyTA420054
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt1 in human liver cell lysate with Akt1 antibody at (A) 1 and (B) 2 ug/ml.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA306225
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Mouse anti-AKT1 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT1 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band (s): none.
Product group Antibodies
OriGene
AKT1 Mouse Monoclonal Antibody [Clone ID: 14E5.A2.B2.H9/E5]TA319564
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetAKT1
  • SizePrice
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA323432 (AKT1 Antibody) at dilution 1/15 (Original magnification: x200)
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA323432
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA323433 (AKT1 Antibody) at dilution 1/25. (Original magnification: x200)
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA323433
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from A549 cells, using AKT1/2/3 (Ab-315/316/312) antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA311645
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from A549 cells, using AKT1/3 (Ab-437/434) antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA313249
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA313259
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
OriGene
AKT1 Rabbit Polyclonal AntibodyTA313262
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (P00024-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (Catalog # P00024-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT1 at approximately 65 kDa. The expected band size for AKT1 is at 56 kDa.
Product group Antibodies
Boster Bio
Anti-Phospho-AKT1 (T450) Rabbit Monoclonal AntibodyP00024-2-30UL
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-Phospho-AKT1 (S129) Rabbit Monoclonal AntibodyP00024-3-30UL
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Product group Antibodies
Boster Bio
Anti-Phospho-AKT1 (S124) Rabbit Monoclonal AntibodyP00024-4-30UL
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
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