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Search results: akt

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Western blot (WB) analysis of akt-1 polyclonal antibody at 1:2000 dilution Lane1:BV2 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt expression in Serum treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt expression in Serum treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis on 293 cell lysate using Akt Antibody
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of Akt in CHO-K1, Jurkat, 3T3, C6 and Hela lysates using Akt antibody.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of AKT in 3T3, C6 and Hela lysates using Akt antibody.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Product group Antibodies
Biorbyt
AKT1 AntibodyORB534096
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetAKT1
  • SizePrice
Western blot analysis of extracts of various cell lines, using Pan-Akt antibody (TA373418) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 30s.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
Western blot analysis of extracts from 293 cells and HepG2 cells, using Akt antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAKT1
  • SizePrice
WB of detection of unphosphorylated and phosphorylated Rabbit Anti-AKT antibody. Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:1000. Secondary antibody: DyLight™ 549 conjugated anti-rabbit IgG (green) and DyLight™ 649 conjugated anti-mouse IgG (red)at 1:10,000.
Product group Antibodies
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityChicken, Human, Mouse, Rat
TargetAKT1
  • SizePrice
Western Blot of Rabbit Anti-AKT Antibody. Lane 1: HeLa cells. Lane 2: H2O2 treated MCF7 cells. Lane 3: Recombinant AKT. Lane 4: A431 cells. Load: 35 ug per lane. Primary antibody: AKT antibody at 1:300 for overnight at 4°C. Secondary antibody: IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature. Block: 1% BLOTTO overnight at 4°C. Predicted/Observed size: ~55-60 kDa for AKT. Other band (s): unspecific.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetAKT1
  • SizePrice
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Akt-T308 antibody (TA373411) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 3s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetAKT1
  • SizePrice