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Search results: anti-Asc

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Product group Antibodies
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetASCL1
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Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetASCC2
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Anti-ASCC2 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
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Anti-ASCC2 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
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Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
TargetASCC3
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Figure 1. Western blotting validation for Anti-ASCC2 Antibody A12860 Western blot analysis of extracts of various cell lines
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetASCC2
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Anti-ASCL1 Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoHistoChemistry
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Anti-ASCL1 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
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Anti-ASCL1 (E158) Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetASCL1
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Figure 1. Western blot analysis of ASCL1 using anti-ASCL1 antibody (A03023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human NCL-H460 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASCL1 antigen affinity purified polyclonal antibody (Catalog # A03023-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASCL1 at approximately 30 kDa. The expected band size for ASCL1 is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-ASCL1 Antibody Picoband(r)A03023-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetASCL1
  • SizePrice
Figure 1. Western blot analysis of ASCL1 using anti-ASCL1 antibody (A03023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human NCL-H460 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASCL1 antigen affinity purified polyclonal antibody (Catalog # A03023-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASCL1 at approximately 30 kDa. The expected band size for ASCL1 is at 25 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetASCL1
  • SizePrice