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Figure 1. Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 24 kDa. The expected band size for ASC/TMS1/PYCARD is at 22 kDa.

Product group Antibodies

Anti-ASC/TMS1/PYCARD Antibody Picoband(r)A00362-6-DYLIGHT594

ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

TargetPYCARD

SizePrice

Product group Antibodies

Goat anti-ASC / TMS1EB05291

ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry

TargetPYCARD

SizePrice

Product group Antibodies

Goat anti-ASC / TMS1, BiotinylatedEB05291-B

ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry

TargetPYCARD

SizePrice

Product group Antibodies

Anti-ASC AntibodyA34511

ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry

SizePrice

Product group Antibodies

Anti-ASC AntibodyA38619

ApplicationsWestern Blot, ImmunoHistoChemistry

SizePrice

Product group Antibodies

Anti-ASC AntibodyA96472

ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry

SizePrice

Product group Antibodies

Anti-ASC AntibodyA48931

ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry

SizePrice

Western Blot Validation in Human HL60 Cells Loading: 15 microg of lysates per lane.Antibodies: ASC A00362, (1 microg/mL) in the absence (A) or presence (B) of blocking peptide, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Product group Antibodies

Anti-ASC PYCARD AntibodyA00362

ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

TargetPYCARD

SizePrice

Product group Antibodies

Anti-ASC / TMS1 Antibody144-64580

ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

TargetPYCARD

SizePrice

Product group Antibodies

Rabbit Anti-ASC/PYCARD Polyclonal AntibodyORB1557650

ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

SizePrice

Product group Antibodies

Anti-ASC/TMS1/Pycard AntibodyORB2588494

ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry

TargetPycard

SizePrice

Product group Antibodies

Anti-ASC/TMS1/Pycard AntibodyORB2588495

ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry

TargetPycard

SizePrice

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