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Figure 1. Western blot analysis of CEA/CEACAM5 using anti-CEA/CEACAM5 antibody (A00356-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA/CEACAM5 antigen affinity purified polyclonal antibody (Catalog # A00356-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA/CEACAM5 at approximately 200 kDa. The expected band size for CEA/CEACAM5 is at 77,180-200 kDa.
Product group Antibodies
Boster Bio
Anti-CEA/CEACAM5 Antibody Picoband(r)A00356-2
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetCEACAM5
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10888-1000
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10889-500
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10890-500
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10891-500
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10892-500
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10893-500
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10894-500
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10895-500
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CEA Antibody130-10896-500
ApplicationsELISA
ReactivityHuman
  • SizePrice
Figure 1. Western blot analysis of CEA using anti-CEA antibody (RP1018). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates, Lane 9: mouse SP20 whole cell lysates, Lane 10: rat RH35 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody (Catalog # RP1018) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.
Product group Antibodies
Boster Bio
Anti-CEACAM5 Antibody Picoband(r)RP1018
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCEACAM5
  • SizePrice
All lanes : Anti-CEA Antibody (Center) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: A549 whole cell lysate Lysates/proteins at 20 microg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product group Antibodies
Boster Bio
Anti-CEA Antibody (Center)A00356-1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCEACAM5
  • SizePrice
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