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Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
Boster Bio
Anti-Interferon gamma/IFNG Antibody Picoband(r)PB9024-CY3
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
  • SizePrice
Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
Boster Bio
Anti-Interferon gamma/IFNG Antibody Picoband(r)PB9024-DYLIGHT488
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
  • SizePrice
Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
Boster Bio
Anti-Interferon gamma/IFNG Antibody Picoband(r)PB9024-DYLIGHT550
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
  • SizePrice
Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
Boster Bio
Anti-Interferon gamma/IFNG Antibody Picoband(r)PB9024-DYLIGHT594
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
  • SizePrice
Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
Boster Bio
Anti-Interferon gamma/IFNG Antibody Picoband(r)PB9024-FITC
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
  • SizePrice
Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
Boster Bio
Anti-Interferon gamma/IFNG Antibody Picoband(r)PB9024-HRP
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
  • SizePrice
Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
Boster Bio
Anti-Interferon gamma/IFNG Antibody Picoband(r)PB9024-IFLUOR647
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
  • SizePrice
Figure 1. Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD.
Product group Antibodies
Boster Bio
Anti-Interferon gamma/IFNG Antibody Picoband(r)PB9024-PE
ApplicationsWestern Blot, ELISA
ReactivityRat
TargetIfng
  • SizePrice
Product group Antibodies
Biomatik
Ifng Polyclonal AntibodyCAC10421
ApplicationsELISA, ImmunoHistoChemistry
ReactivityMouse
TargetIfng
  • SizePrice
Product group Antibodies
Biomatik
Ifng Polyclonal AntibodyCAC07063
ApplicationsWestern Blot, ELISA
ReactivityGuinea Pig
TargetIfng
  • SizePrice
Product group Proteins / Signaling Molecules
7BioScience
Anti-Murine IFN-gamma, biotin7B-AMIFNGB
  • SizePrice
Product group Antibodies
7BioScience
Anti-Human IFN-gamma, biotin7B-AHIFNGB
  • SizePrice
7891011

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