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Figure 1. Western blot analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (A00214-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 Receptor alpha/Il2ra antigen affinity purified polyclonal antibody (Catalog # A00214-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 Receptor alpha/Il2ra at approximately 50 kDa. The expected band size for IL-2 Receptor alpha/Il2ra is at 50 kDa.
Product group Antibodies
Boster Bio
Anti-IL-2 Receptor alpha/Il2ra Antibody Picoband(r)A00214-1
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetIl2ra
  • SizePrice
Protein structure of IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0234).
Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
IL-2 (human) (Switch-2) (monomeric):Fc-KIH (human) (rec.)AG-40B-0234
  • SizePrice
IL-2 (R58A, F62A, Y65A, E82A, C145A) (Woodchuck) Recombinant
Product group Proteins / Signaling Molecules
BPS Bioscience
IL-2 (R58A, F62A, Y65A, E82A, C145A) (Woodchuck) Recombinant100156
  • SizePrice
Human IL-2 Receptor alpha Matched Antibody Pair Kit
Product group Antibodies
Antibodies.com
Human IL-2 Receptor alpha Matched Antibody Pair KitA325343
ApplicationsELISA
ReactivityHuman
  • SizePrice
Human IL-2 Receptor alpha Matched Antibody Pair Kit
Product group Antibodies
Antibodies.com
Human IL-2 Receptor alpha Matched Antibody Pair KitA325344
ApplicationsELISA
ReactivityHuman
  • SizePrice
Protein Structure
Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
IL-2 (mouse) (monomeric):Fc-KIH (human) (rec.)AG-40B-0225
  • SizePrice
Product group Assays
Biorbyt
Human IL-2 ELISpot Kit 5 x 96 (pre-coated plates)ORB2705636-4
ReactivityHuman
  • SizePrice
Protein Structure
Product group Proteins / Signaling Molecules
AdipoGen Life Sciences
IL-2 Superkine (monomeric):Fc-KIH (human) (rec.)AG-40B-0222
  • SizePrice
Product group Proteins / Signaling Molecules
Biorbyt
IL-2 Protein, Rhesus macaque, Recombinant (His & Myc & SUMO)ORB1977478
Protein IDP68291
  • SizePrice
IL-2 Protein, Rhesus macaque, Recombinant (His & Myc & SUMO)
Product group Proteins / Signaling Molecules
TargetMol Chemicals
IL-2 Protein, Rhesus macaque, Recombinant (His & Myc & SUMO)TMPH-02442
  • SizePrice
Product group Proteins / Signaling Molecules
Biorbyt
IL-2 R beta/CD122 Protein, Human, Recombinant (His & Avi)ORB1960567
Protein IDP14784
  • SizePrice
IL-2 R beta/CD122 Protein, Human, Recombinant (His & Avi)
Product group Proteins / Signaling Molecules
TargetMol Chemicals
IL-2 R beta/CD122 Protein, Human, Recombinant (His & Avi)TMPK-00320
  • SizePrice
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