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Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-BIOTIN
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-CY3
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-DYLIGHT488
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-DYLIGHT550
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-DYLIGHT594
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-FITC
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-HRP
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-IFLUOR647
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1-PE
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Figure 1. Western blot analysis of IL23 using anti-IL23 antibody (A01097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PANC-1 cell lysates, Lane 3: rat lymph tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 antigen affinity purified polyclonal antibody (Catalog # A01097-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 at approximately 19KD. The expected band size for IL23 is at 21KD.
Product group Antibodies
References
Boster Bio
Anti-IL23/IL23A Antibody Picoband(r)A01097-1
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetIL23A
  • SizePrice
Linker-free, recombinant Human IL-23 was resolved by electrophoresis, transferred to nitrocellulose and probed with the anti-IL23 p19 specific HLT2736 antibody. Proteins were visualized using a Goat anti-Mouse secondary conjugated to HRP and a chromogen.
Product group Antibodies
OriGene
IL23 (IL23A) Mouse Monoclonal Antibody [Clone ID: HLT2736]AM20386PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetIL23A
  • SizePrice
2728293031

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