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Search results: p53 TP53

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
p53 (TP53) Mouse Monoclonal Antibody [Clone ID: OTI1F4]CF502802
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Product group Antibodies
OriGene
p53 (TP53) Rabbit Monoclonal Antibody [Clone ID: RM387]TA398569
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Immunohistochemistry staining of Paraffin Colon carcinoma tissue by p53 antibody (dilution: 1:100 - 1:500; visualization of staining: Nuclear)
Product group Antibodies
OriGene
p53 (TP53) Mouse Monoclonal Antibody [Clone ID: DO7]TA327719
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
Product group Antibodies
OriGene
p53 (TP53) Mouse Monoclonal Antibody [Clone ID: PAb421]TA385747
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rabbit, Rat
TargetTP53
  • SizePrice
Western Blot using anti-p53 antibody PAb421 (TA385748). A431 cell nuclear (A) and cytoplasmic (B) extract (35microg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (TA385748) at 0.1 microg/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). TA385748 successfully detected both human nuclear and cytoplasmic p53.
Product group Antibodies
OriGene
p53 (TP53) Rabbit Monoclonal Antibody [Clone ID: PAb421]TA385748
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rabbit, Rat
TargetTP53
  • SizePrice
IHC: The human breast cancer tissue stained with Mouse anti-P53 monoclonal antibody at 1:500. (Note: formalin-fixed, paraffin-embedded sections need 15 minutes heatinduced epitope retrieval in 10 mM citrate buffer, pH 6.0, and 30 minutes incubation at room temperature with the primary antibody.
Product group Antibodies
OriGene
p53 (TP53) Mouse Monoclonal Antibody [Clone ID: S20]TA354364
ApplicationsImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTP53
  • SizePrice
MCF-7 cells were treated with 50 uM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with rabbit polyclonal antibody against acetylated p53 (Lys382). Lane 1=untreated, control MCF-7 cells; Lane 2=treated MCF-7 cells.
Product group Antibodies
OriGene
p53 (TP53) Rabbit Polyclonal Antibody [Clone ID: Poly6142]TA327919
ApplicationsWestern Blot
ReactivityHuman
TargetTP53
  • SizePrice
Product group Proteins / Signaling Molecules
MedChem Express
Cellular tumor antigen P53/TP53, Human (His-SUMO)HY-P72257
Protein IDP04637
  • SizePrice
More sizes
TP53 activity in the over-expression lysate (LY400186) was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the over-expression lysate and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm. Overexpression cell lysates are prepared from HEK293T cells transfected with RC200003 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
p53 (TP53) (NM_000546) Human Over-expression LysateLY400186
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY426652) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225604 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
p53 (TP53) (NM_001126112) Human Over-expression LysateLY426652
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY426653) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225518 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
p53 (TP53) (NM_001126113) Human Over-expression LysateLY426653
  • SizePrice
Western blot validation of overexpression lysate (Cat# LY426654) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225502 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
p53 (TP53) (NM_001126114) Human Over-expression LysateLY426654
  • SizePrice
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