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Search results: p53 TP53

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Product group Antibodies
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Product group Antibodies
MyBioSource
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Figure 1. Western blot analysis using p53 antibody at 10 ug/ml on HCT116 cell lysate (Lane 1), HCT116 cell lysate activated with adriamycin (Lane 2), p21-/- cell lysate (Lane 3), P21-/- cell lysate activated with ADR (Lane 4) and p53-/- activated with ADR (Lane 5). ADR activates p53 in cells. Also shown is a graphic representation of the epitope location.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ELISA
ReactivityHuman, Mouse, Xenopus
TargetTP53
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Figure 1. Western blot analysis using p53 antibody at 1 ug/ml on native H1299 cells (Lane 1) and H1299 cells transfected with human p53 (Lane 2).
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetTP53
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Detection of activated p53 Recombinant C-terminal fragment of p53 was incubated with Casein Kinase II in the absence (1) or presence (2) of ATP. Proteins were separarted by SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Monoclonal p53 antibody (clone 9F4) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
Product group Antibodies
OriGene
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetTP53
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Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). A - anti-p53 (total) B- anti-p53 (phospho Ser392) Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetTP53
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetTP53
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetTP53
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetTP53
  • SizePrice