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Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-ERG Antibody Picoband(r)A00793-1-DYLIGHT488
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-ERG Antibody Picoband(r)A00793-1-DYLIGHT550
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-ERG Antibody Picoband(r)A00793-1-DYLIGHT594
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-ERG Antibody Picoband(r)A00793-1-FITC
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-ERG Antibody Picoband(r)A00793-1-HRP
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-ERG Antibody Picoband(r)A00793-1-IFLUOR647
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-ERG Antibody Picoband(r)A00793-1-PE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
Figure 1. Western blot analysis of ERG using anti-ERG antibody (A00793-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERG antigen affinity purified polyclonal antibody (Catalog # A00793-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERG at approximately 55 kDa. The expected band size for ERG is at 55 kDa.
Product group Antibodies
Boster Bio
Anti-ERG Antibody Picoband(r)A00793-1
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
Figure 1. Staining ERG in Prostate by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Product group Antibodies
OriGene
ERG (C-term) Goat Polyclonal AntibodyAP07971PU-N
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey
TargetERG
  • SizePrice
Immunohistochemistry: (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Product group Antibodies
OriGene
ERG (C-term) Goat Polyclonal AntibodyAP16447PU-N
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ERG (Cat# RC208093, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERG(Cat# TA506076). Positive lysates [LY403650] (100ug) and [LC403650] (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ERG Mouse Monoclonal Antibody [Clone ID: OTI4H7]CF506076
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERG (RC208093, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERG. Positive lysates LY403650 (100ug) and LC403650 (20ug) can be purchased separately from OriGene.
Product group Antibodies
OriGene
ERG Mouse Monoclonal Antibody [Clone ID: OTI5F12]CF506077
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetERG
  • SizePrice
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