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ProductsBack/SEC13 Antibody (aa72-100)

SEC13 Antibody (aa72-100)

LS-C168924
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetSEC13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SEC13 Antibody (aa72-100)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    Flo (1:10 - 1:50), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6396
  • Target name
    SEC13
  • Target description
    SEC13 homolog, nuclear pore and COPII coat complex component
  • Target synonyms
    D3S1231E; GATOR complex protein SEC13; npp-20; protein SEC13 homolog; SEC13 homolog, nuclear pore and COPII coating complex component; SEC13L1; SEC13-like protein 1; SEC13R; SEC13-related protein
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEC13L1/SEC13 using anti-SEC13L1/SEC13 antibody (A04346-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat small intestine tissue lysates, Lane 7: mosue brain tissue lysates, Lane 8: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC13L1/SEC13 antigen affinity purified polyclonal antibody (Catalog # A04346-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC13L1/SEC13 at approximately 36 kDa. The expected band size for SEC13L1/SEC13 is at 36 kDa.
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