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ProductsBack/SEC23A Antibody
Immunofluorescent analysis of Hela cells using CSB-PA618985LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using CSB-PA618985LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells using CSB-PA618985LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

SEC23A Antibody

CSB-PA618985LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetSEC23A
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEC23A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10484
  • Target name
    SEC23A
  • Target description
    SEC23 homolog A, COPII component
  • Target synonyms
    CLSD, hSec23A, protein transport protein Sec23A, SEC23 homolog A, COPII coat complex component, SEC23-related protein A, Sec23 homolog A, coat complex II component
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15436
  • Protein Name
    Protein transport protein Sec23A
  • Scientific Description
    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SEC23A using anti-SEC23A antibody (A05287-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC23A antigen affinity purified polyclonal antibody (Catalog # A05287-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC23A at approximately 86KD. The expected band size for SEC23A is at 86KD.
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