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SEC61B antibody

GTX129852
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetSEC61B
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Overview

  • Supplier
    GeneTex
  • Product Name
    SEC61B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.77 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10952
  • Target name
    SEC61B
  • Target description
    SEC61 translocon subunit beta
  • Target synonyms
    protein transport protein Sec61 subunit beta, SEC61 translocon beta subunit, Sec61 beta subunit, Sec61 complex, beta subunit, protein translocation complex beta, protein transport protein SEC61 beta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60468
  • Protein Name
    Protein transport protein Sec61 subunit beta
  • Scientific Description
    The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chen MK, Du Y, Sun L, et al. H(2)O(2) induces nuclear transport of the receptor tyrosine kinase c-MET in breast cancer cells via a membrane-bound retrograde trafficking mechanism. J Biol Chem. 2019,294(21):8516-8528. doi: 10.1074/jbc.RA118.005953
    Read this paper

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Figure 1. Western blot analysis of SEC61B using anti-SEC61B antibody (A07334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse C2C12 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC61B antigen affinity purified polyclonal antibody (Catalog # A07334-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC61B at approximately 15 kDa. The expected band size for SEC61B is at 10 kDa.
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