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Immunohistochemical staining of human pancreas shows moderate to strong positivity in endoplasmic reticulum in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate to strong positivity in endoplasmic reticulum in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate to strong positivity in endoplasmic reticulum in exocrine glandular cells.

Anti-SEC61B Antibody

HPA049407
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSEC61B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SEC61B Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10952
  • Target name
    SEC61B
  • Target description
    SEC61 translocon subunit beta
  • Target synonyms
    protein transport protein Sec61 subunit beta, SEC61 translocon beta subunit, Sec61 beta subunit, Sec61 complex, beta subunit, protein translocation complex beta, protein transport protein SEC61 beta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60468
  • Protein Name
    Protein transport protein Sec61 subunit beta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SEC61B using anti-SEC61B antibody (A07334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse C2C12 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC61B antigen affinity purified polyclonal antibody (Catalog # A07334-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC61B at approximately 15 kDa. The expected band size for SEC61B is at 10 kDa.
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